Application Brief #012
Automated Spreeta Analysis System
(PDF, 545K, 5 pages)
Current as of June 2003
The data quality that is attainable using Spreeta is, in part, a function of the peripheral technologies that are employed. The latest Spreeta designs that we have produced yield low signal noise and the flow cell can be made to dimensions similar to the Biacore instruments...

--> Application Brief #008
Competitive Assay: Determination of Biotin Concentration
(PDF, 667K, 10 pages)
Current as of June 2003
A competitive assay format is demonstrated for the determination of biotin using Spreeta technology. In this assay biotinylated bovine serum albumin is physisorbed directly to the gold surface. Solutions containing a fixed concentration of antibody and variable concentrations of free biotin are premixed and analyzed.

Application Brief #006
Affinity Constant Estimation
(PDF, 561K, 6 pages)
Current as of June 2003
This note outlines solution phase affinity analysis of the interaction of an anti–dinitrophenyl monoclonal antibody with dinitrophenyl. The affinity constant is calculated by fitting an equilibrium model to a plot of free antibody concentration as a function of the DNP concentration in equilibrium mixtures.

Application Brief #005
Direct Assay: Detection of Mouse IgG
(PDF, 563K, 6 pages)
Current as of June 2003
Direct Assay for the Detection of Mouse IgG using Spreeta. Protein A is immobilized and standard mouse IgG solutions are analyzed.

Application Brief #004
On the Sensitivity of Spreeta
(PDF, 733K, 14 pages)
Current as of June 2003
A competitive assay format is demonstrated for the determination of biotin using Spreeta technology. In this assay biotinylated bovine serum albumin is physisorbed directly to the gold surface. Solutions containing a fixed concentration of antibody and variable concentrations of free biotin are premixed and analyzed.

Application Brief #003
Ligand Immobilization: Method 3
(PDF, 550K, 5 pages)
Current as of June 2003
In this method a thiolated ligand is linked to a gold surface that is pre–coated with pyridyl disulfide groups. The ligand is covalently linked to the surface by disulfide exchange. It is possible to regenerate this surface using disulfide reductants.

Application Brief #002
Immobilization of Ligand: Method 2 (Avidin-biotin Ligand Immobilization)
(PDF, 640K, 8 pages)
Current as of June 2003
In this method the ligand is linked to the gold surface using the avidin-biotin affinity system.

Application Brief #001
Immobilization of Ligand: Method 1
(PDF, 831K, 6 pages)
Current as of June 2003
In this method the ligand is linked to the gold surface via a cross–linked protein film using carbodiimide mediated amide bond formation.